2013. IDENTIFICATION OF GENE RESISTANCE TO AVIAN INFLUENZA VIRUS (MX GENE) AMONG WILD WATERBIRDS. Makara Journal of Science 17(1): 6-10. Retno Damayanti Soejoedono, Sri Murtini

IDENTIFICATION OF GENE RESISTANCE TO AVIAN INFLUENZA VIRUS (MX GENE) AMONG WILD WATERBIRDS.

Dewi Elfidasari, Dedy Duryadi Solihin, Retno Damayanti Soejoedono, Sri Murtini

ABSTRACT

The Mx gene is an antiviral gene used to determine the resistance or the susceptibility to different types of viruses, including the Avian Influenza (AI) virus subtype H5N1. The AI virus subtype H5N1 infection in chickens causes Mx gene polymorphism. The Mx+ gene shows resistant to the AI virus subtype H5N1, whereas the Mx gene shows signs of susceptible. The objective of this research was to detect the Mx gene in wild aquatic birds using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method with the primer pairs F2 and NE-R2/R and the RsaI restriction enzyme. DNA samples were obtained from eight species of wild water birds with positive and negative exposure to the AI virus subtype H5N1. DNA amplification results showed that the Mx gene in wild aquatic birds is found in a 100 bp fragment, which is the same as the Mx gene found in chickens. However, unlike chickens, the Mx gene in wild aquatic birds did not show any polymorphism. This study proves that Mx- based resistance to AI virus subtype H5N1 in different in wild birds than in chickens.

ABSTRAK

Identifikasi Gen Mengatur Resistensi terhadap Virus AI (Gen Mx) pada Burung Air Liar Penetap di Cagar Alam Pulau Dua Serang, Provinsi Banten. Gen Mx merupakan gen antiviral yang berperan menentukan sifat resisten atau rentan terhadap berbagai jenis virus, termasuk virus AI subtipe H5N1. Infeksi virus AI subtipe H5N1 pada ayam mengakibatkan polimorfisme gen Mx. Gen Mx+ menunjukkan sifat resisten sedangkan gen Mx- menunjukan sifat rentan terhadap virus AI subtipe H5N1. Tujuan penelitian adalah membuktikan bahwa gen Mx terdapat pada burung air liar dan terdapat polimorfisme gen Mx pada burung air liar akibat paparan virus AI subtipe H5N1. Metode deteksi gen Mx menggunakan PCR-RFLP dengan sepasang primer NE-F2 dan NE-R2/R dan enzim pemotongan RsaI. Sampel DNA burung air liar berasal dari 8 jenis burung air liar yang positif dan negatif terhadap paparan virus AI subtipe H5N1. Hasil amplifikasi DNA menunjukkan bahwa gen Mx pada burung air liar dijumpai pada panjang fragmen 100bp, sama seperti gen Mx yang terdapat ayam. Akan tetapi gen Mx pada burung air liar tidak menunjukan adanya polimorfisme seperti yang dijumpai pada ayam. Penelitian ini membuktikan bahwa keberadaan gen Mx pada burung air liar dalam mempengaruhi mekanisme resistensi terhadap paparan virus AI subtipe H5N1 berbeda dengan yang terdapat pada ayam.

Keywords: Mx gene, wild waterbirds, exposure, AI virus subtype H5N1, resistance

Publish in Makara Journal of Science 17/1 (2013) 6-10

 

0 replies

Leave a Reply

Want to join the discussion?
Feel free to contribute!

Leave a Reply

Your email address will not be published. Required fields are marked *