Sonni Martahadi. UJI POTENSI EKSTRAK TEMU IRENG ( Curcuma aeroginosa Roxb. ) PADA AYAM BROILER YANG DIINFEKSI VIRUS AVIAN INFLUENZA H5N1: HISTOPATOLOGI HATI DAN GINJAL. B04063532. Skripsi. 2010. Pembimbing: Wiwin Winarsih, Agus Setiyono

UJI POTENSI EKSTRAK TEMU IRENG ( Curcuma aeroginosa Roxb. ) PADA AYAM BROILER YANG DIINFEKSI VIRUS AVIAN INFLUENZA H5N1: HISTOPATOLOGI HATI DAN GINJAL

(The Potency Test of Black Turmeric (Curcuma eroginosa Roxb.) in Broiler were Infected with H5N1 Avian Influenza Virus: Histopathology of Liver and Kidney)

SONNI MARTAHADI, WIWIN WINARSIH, AGUS SETIYONO

RINGKASAN

Avian Influenza merupakan salah satu penyakit zoonotik yang sangat berbahaya dan belum dapat diatasi secara optomal. Penelitian ini bertujuan untuk mengetahui potensi ekstrak temu ireng (Curcuma aeroginosa Roxb.) dalam menanggulangi infeksi virus H5N1 melalui histopatologi hati dan ginjal. Penelitian ini menggunakan ayam Broiler dari strain Cobb. Yang dibagi menjadi 4 kelompok, yaitu K1 (diberi ekstrak), K2 ( tanpa perlakuan), P1 (diberi ekstrak dan diinfeksi virus), P2 (diinfeksi virus). Ekstrak temu ireng diberikan pada ayam selama 3 minggu dalam kandang sistem postal dan kemudian ayam diinfeksi virus H5N1 dalam fasilitas Bio Safety Laboratory level 3 (9BSL 3). Data kuantitatif yang diperoleh dari skoring kemudian dianalisa menggunakan Uji Kruskal Wallis dan Uji Dunn. Hasil penelitian menunjukkan bahwa kematian ayam yang diberi ekstrak temu ireng dan diinfeksi virus H5N1 dapat dihambat. Selain itu terjadi pengurangan persentasi lesio histopatologi pada hati berupa hemoragi, infiltrasi sel radang, dan fokus nekrotik, serta pada ginjal terjadi pengurangan persentasi lesio histopatologi berupa infiltrasi sel radang dan fokus nekrotik.

Kata kunci: Ekstrak temu ireng, virus H5N1, ayam Broiler, hati, ginjal, histopatologi.

ABSTRACT

Avian Influenza is one of the zoonotic disease which is very dangerous and not yet to be controlled optimally. The aim of this resarch was to observe the potency of black turmeric (Curcuma aeroginosa Roxb.) extract in preventing the infection of H5N1 virus through liver and kidney histopathologycal discriptions. This research used Cobb strain of Broiler which were devided into 4 groups included K1 (extract treatment), K2 (no extract treatment and no virus infection), P1 (extract treatment and virus infection), and P2 (virus infection). Black turmeric extract was given to Broiler during 3 weeks in postal system cage and then the Broiler were infected by H5N1 virus in Biosafety Laboratory level 3 facilities. The quantitative data obtained by scoring and then were analyzed by Kruskal Wallis and Dunn test. The result revealed that the death of Broiler which were treated with Curcuma aeroginosa Roxb. extract and were infected with H5N1 virus could be obstructed. The histopathological lesions such as hemorrhage, inflammatory focuse, necrose in liver, and also inflammatory focuse and necrose in kidney could be reduced.

Key words: Black turmeric extract, H5N1 virus, Broiler chicks, liver, kidney, histopathology

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